![]() ![]() Most results are available within five days. HSV Western blot serologies are run three times a week. Since fewer than 5% of patients demonstrate a detectable rise in antibody titer during recurrent HSV episodes, an interpretive report is given instead of numerical values. In addition, Western blot readily documents seroconversion. The specificity of the Western blot assay provides an accurate distinction in 99% of patients between antibody to HSV-1 and antibodies to HSV-2. ![]() ![]() Our laboratory detects HSV Antibodies by Western blot assay, which is not only highly sensitive for detecting HSV antibodies, but also is highly accurate in differentiating past HSV-1 from HSV-2 infections and determining whether someone has antibodies to both viruses. However, serologies for HSV are useful in determining whether a person has had a past infection with HSV-1 or HSV-2, and is the best way to detect “silent carriers” of HSV-2. Viral isolation and subsequent subtyping is generally the best way to document an acute HSV infection. Search HBCA code in the online Laboratory Test Catalog for information on Hepatitis B Core Antibody (HBcAb). Search HBSA code in the online Laboratory Test Catalog for information on Hepatitis B Surface Antibody (HBsAb). Anti-HBs assays and anti-HBc assays are performed daily Monday through Friday. Vaccination/re-vaccination is warranted with values below this level. of Hepatitis B surface antibody are considered protective levels. Values above a test standard containing 10 I.U. The Hepatitis B surface antibody result is reported international units (I.U.) of Hepatitis B surface antibody. needle stick exposures), for documenting persons with past infection with Hepatitis B virus, for vaccine screening, and for determining response to vaccination. Hepatitis B surface antibody (anti-HBs) and Hepatitis B core antibody (anti-HBc) assays are useful for identifying persons susceptible to Hepatitis B infection (i.e. See Hepatitis B Chart 1, Chart 2, and Chart 3 Search HBB code in the online Laboratory Test Catalog for more information on Hepatitis B Battery (HBSAb, HBSAg, and HBCAb). Search HBSS code in the online Laboratory Test Catalog for more information on Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb). Search HBSAG or HBSAGX code in the online Laboratory Test Catalog. Note: Positive HBsAg will reflex to a Hepatitis B DNA by PCR at an additional charge. The physician must also report acute Hepatitis B infections to the local County Department of Public Health (in King County, telephone the Communicable Disease Department at (206) 296-4774). The routine HBsAg run can accommodate most specimens generated by accidental parenteral exposure. Patients with needle sticks and other parenteral exposures need to receive Hepatitis B Immune Globulin within 72 hours of exposure. Reports of reactive HBsAg are called to the ordering physician or laboratory and are automatically run for Hepatitis B viral DNA by PCR. Infectivity of a patient is determined by enzyme immunoassay for Hepatitis B surface antigen (HBsAg), which is run Monday through Friday. Contact the University of Washington Clinical Virology Lab (206 685-8037) for ordering instructions.Hepatitis B is transmitted through blood or secretions of infected patients. CSF antibody testing will be performed ONLY when paired with a serum sample. For accurate seroconversion determination, the acute and convalescent samples should be drawn at least 12 -16 weeks apart.įor CSF, see HSV SemiQnt Rapid PCR, Swab/CSF (Herpes Simplex Virus by PCR). It has not been cleared or approved by the US Food and Drug Administration.įor Paired samples, see Herpes Simplex Seroconversion Panel by Western Blot (Paired) for seroconversion on paired serum samples. This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. HSV2 - Positive, Negative or Indeterminate.HSV1 - Positive, Negative or Indeterminate.The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins to which the patient's immune system has antibody. Antibodies, which bind to the viral proteins, are detected by an enzyme-mediated color change. The strips of paper or "blots" containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. HSV1 and HSV2 proteins from detergent lysates ("Bernstein's lysate") of HSV infected cells are separated by electrophoresis and transferred to nitrocellulose paper. The detection of HSV1 and HSV2 IgG class antibodies by Western blot in serum or CSF.
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